Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle
نویسندگان
چکیده
منابع مشابه
Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle.
We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an opti...
متن کاملFast optically sectioned fluorescence HiLo endomicroscopy.
We describe a nonscanning, fiber bundle endomicroscope that performs optically sectioned fluorescence imaging with fast frame rates and real-time processing. Our sectioning technique is based on HiLo imaging, wherein two widefield images are acquired under uniform and structured illumination and numerically processed to reject out-of-focus background. This work is an improvement upon an earlier...
متن کاملOptically sectioned in vivo imaging with speckle illumination HiLo microscopy.
We present a simple wide-field imaging technique, called HiLo microscopy, that is capable of producing optically sectioned images in real time, comparable in quality to confocal laser scanning microscopy. The technique is based on the fusion of two raw images, one acquired with speckle illumination and another with standard uniform illumination. The fusion can be numerically adjusted, using a s...
متن کاملWhole-field optically sectioned fluorescence lifetime imaging.
We describe a novel three-dimensional fluorescence lifetime imaging microscope that exploits structured illumination to achieve whole-field sectioned fluorescence lifetime images with a spatial resolution of a few micrometers.
متن کاملFluorescence endomicroscopy with structured illumination.
We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (approximately 2.6 microm) optically sectioned fluorescence images over a field of view of about 240 microm. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifact...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2009
ISSN: 1083-3668
DOI: 10.1117/1.3130266